Virus:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Bioprocessing:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Recombinant:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Blocking Assay:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Staining:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Clinical Proteomics:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
Gene Expression:Article Title: Therapeutic VEGFC treatment provides protection against traumatic-brain-injury-driven tauopathy pathogenesis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Phospho-Tau (Ser202, Thr205) (AT8) biotin-conjugated Invitrogen Cat# MN1020B; RRID:AB_223648 MC1 P. Davies Albert Einstein College of Medicine; New York; USA Cat # MC1; RRID: AB_2314773 Goat polyclonal anti-IBA1 Abcam Cat# ab5076; RRID:AB_2224402 Rabbit monoclonal anti-P2RY12 (clone EPR26298-93) Abcam Cat# ab300140; RRID:AB_3677650 Rat monoclonal anti-MHC class II Invitrogen Cat# 14-5321-82; RRID:AB_467561 Rabbit monoclonal anti-synaptophysin (clone YE269) Abcam Cat# 32127; RRID:AB_2286949 Rat monoclonal anti-Lyve1(clone ALY7) Invitrogen Cat# 50-0443-82; RRID:AB_10597449 Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID:AB_305564 Mouse monoclonal anti-NeuN biotinconjugated (clone A60) Millipore Sigma Cat# MAB377B; RRID:AB_177621 Bacterial and virus strains AAV1-CMV-mVEGFC-WPRE Vector Biolabs Cat# AAV-275994; RefSeq ID NM_009506; Lot# 210405–220318, 230605#4 AAV1-CMV-eGFP Vector Biolabs Cat# 7002; Lot# 220314#19, 230605#3 Chemicals, peptides, and recombinant proteins Urea Sigma-Aldrich Cat# U5128 N,N,N ′ ,N ′ -Tetrakis(2-Hydroxypropyl) ethylenediamine, Quadrol® Sigma-Aldrich Cat# 122262 Triton X-100 Sigma-Aldrich Cat# X100 Triethanolamine Sigma-Aldrich Cat# 90279 Mineral oil Sigma Aldrich Cat# 330779 Tissue-Tek OCT compound Sakura Cat# 4583 Mouse on mouse blocking reagent Vector Laboratories Cat# MKB-2213-1 NeuroTrace 500/525 Fluorescent Nissl Stain Invitrogen Cat# N21480 DAPI Sigma Aldrich Cat# D9542-1MG ProLong Gold Antifade Mountant Invitrogen Cat# P36930 True Black Lipofusin Autofluorescence Quencher, 20X in DMF Biotium Cat# 23007 FluoSpheres carboxylate 0.5mm (beads) 505/515 Invitrogen Cat# F8813 artificial cerebrospinal fluid (CSF) Harvard Apparatus UK Cat# 597316 Deposited data Gene expression data for replication This paper GEO: GSE281850, https://www.ncbi.nlm. nih.gov/geo/query/acc.cgi?acc=GSE281850 Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.17180220 GitHub: https://github.com/LukensLab/ TBI_snRNA-seq Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX Stock no. 000664; RRID:IMSR_JAX:000664 B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J The Jackson Laboratory JAX Stock no. 008169 RRID:IMSR_JAX:008169 (Continued on next page) Cell Reports 44, 116521, November 25, 2025 11 ..
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